Single-Cell Imaging Reveals That Staphylococcus aureus Is Highly Competitive Against Pseudomonas aeruginosa on Surfaces

被引:7
|
作者
Niggli, Selina [1 ]
Wechsler, Tobias [1 ]
Kuemmerli, Rolf [1 ]
机构
[1] Univ Zurich, Dept Quantitat Biomed, Zurich, Switzerland
基金
欧盟地平线“2020”;
关键词
opportunistic human pathogens; single-cell microscopy; surface colonization; interspecies interactions; polymicrobial infections; PHENOL-SOLUBLE MODULINS; DETERMINANTS; INFECTIONS; VIRULENCE; GROWTH;
D O I
10.3389/fcimb.2021.733991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pseudomonas aeruginosa and Staphylococcus aureus frequently occur together in polymicrobial infections, and their interactions can complicate disease progression and treatment options. While interactions between P. aeruginosa and S. aureus have been extensively described using planktonic batch cultures, little is known about whether and how individual cells interact with each other on solid substrates. This is important because both species frequently colonize surfaces to form aggregates and biofilms in infections. Here, we performed single-cell time-lapse fluorescence microscopy, combined with automated image analysis, to describe interactions between P. aeruginosa PAO1 with three different S. aureus strains (Cowan I, 6850, JE2) during microcolony growth on agarose surfaces. While P. aeruginosa is usually considered the dominant species, we found that the competitive balance tips in favor of S. aureus on surfaces. We observed that all S. aureus strains accelerated the onset of microcolony growth in competition with P. aeruginosa and significantly compromised P. aeruginosa growth prior to physical contact. Upon direct contact, JE2 was the most competitive S. aureus strain, simply usurping P. aeruginosa microcolonies, while 6850 was the weakest competitor itself suppressed by P. aeruginosa. Moreover, P. aeruginosa reacted to the assault of S. aureus by showing increased directional growth and expedited expression of quorum sensing regulators controlling the synthesis of competitive traits. Altogether, our results reveal that quantitative single-cell live imaging has the potential to uncover microbial behaviors that cannot be predicted from batch culture studies, and thereby contribute to our understanding of interactions between pathogens that co-colonize host-associated surfaces during polymicrobial infections.
引用
收藏
页数:13
相关论文
共 50 条
  • [41] Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa
    Lima, Marta
    Teixeira-Santos, Rita
    Gomes, Luciana C.
    Faria, Sara, I
    Valcarcel, Jesus
    Antonio Vazquez, Jose
    Cerqueira, Miguel A.
    Pastrana, Lorenzo
    Bourbon, Ana, I
    Mergulhao, Filipe J.
    MOLECULES, 2021, 26 (14):
  • [42] Effect of Electrical Current on the Activities of Antimicrobial Agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis Biofilms
    del Pozo, Jose L.
    Rouse, Mark S.
    Mandrekar, Jayawant N.
    Sampedro, Marta Fernandez
    Steckelberg, James M.
    Patel, Robin
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (01) : 35 - 40
  • [43] Photodynamic therapy using methylene blue, combined or not with gentamicin, against Staphylococcus aureus and Pseudomonas aeruginosa
    Perez-Laguna, Vanesa
    Garcia-Luque, Isabel
    Ballesta, Sofia
    Perez-Artiaga, Luna
    Lampaya-Perez, Veronica
    Rezusta, Antonio
    Gilaberte, Yolanda
    PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2020, 31
  • [44] In vitro postantibiotic effects of tomopenem (CS-023) against Staphylococcus aureus and Pseudomonas aeruginosa
    Tomozawa, Takanori
    Sugihara, Chika
    Kakuta, Masayo
    Sugihara, Kiyoshi
    Koga, Tetsufumi
    JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (04) : 438 - 441
  • [45] Antibiofilm Activity of Antarctic Sponge-Associated Bacteria against Pseudomonas aeruginosa and Staphylococcus aureus
    Rizzo, Carmen
    Zammuto, Vincenzo
    Lo Giudice, Angelina
    Rizzo, Maria Giovanna
    Spano, Antonio
    Lagana, Pasqualina
    Martinez, Miguel
    Guglielmino, Salvatore
    Gugliandolo, Concetta
    JOURNAL OF MARINE SCIENCE AND ENGINEERING, 2021, 9 (03) : 1 - 16
  • [46] Activity of disinfectants against multispecies biofilms formed by Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa
    Kart, Didem
    Tavernier, Sarah
    Van Acker, Heleen
    Nelis, Hans J.
    Coenye, Tom
    BIOFOULING, 2014, 30 (03) : 377 - 383
  • [47] In vitro antagonism of ethanolic extracts from Tabernaemontana against Staphylococcus aureus and Pseudomonas aeruginosa strains
    Barbaran Urresti, Cecilia De Fatima
    Rios Rios, Jorge Manases
    Bendayan Acosta, Maria Elena
    Heredia Quezada, Gregorio
    Tresierra-Ayala, Alvaro
    CIENCIA AMAZONICA, 2021, 9 (02): : 9 - 20
  • [48] Activity of combinations of ceftazidime, imipenem and pefloxacin against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa
    El-Sukhon, SN
    Boukhatem, ZF
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2003, 22 (06) : 613 - 617
  • [49] COMPARISON OF EFFICACY OF FIVE VARIOUS COMMERCIAL PRODUCTS OF CIPROFLOXACIN AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA
    Shrivastav, Vivek Kumar
    Shukla, Deepali
    Jana, Ashamukul
    Shrivastav, Archana
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2013, 4 (02): : 731 - 733
  • [50] In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus
    Robinson, Duane A.
    Griffith, Ronald W.
    Shechtman, Dan
    Evans, Richard B.
    Conzemius, Michael G.
    ACTA BIOMATERIALIA, 2010, 6 (05) : 1869 - 1877