Molecular cloning and characterisation of a novel P-glycoprotein in the salmon louse Lepeophtheirus salmonis

被引:32
|
作者
Heumann, Jan [1 ]
Carmichael, Stephen [1 ]
Bron, James E. [1 ]
Tildesley, Andy [2 ,3 ]
Sturm, Armin [1 ]
机构
[1] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
[2] Univ Stirling, Marine Environm Res Lab, Machrihanish PA28 6PZ, Argyll, Scotland
[3] Univ Stirling, Marine Environm Res Lab, Machrihanish PA28 6PZ, Bute, Scotland
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2012年 / 155卷 / 02期
关键词
ABC transporter; Sea louse; Emamectin benzoate; SLICE (R); Drug resistance; MULTIXENOBIOTIC RESISTANCE MECHANISM; TRANSPORTER GENE FAMILY; SEA LICE; MULTIDRUG-RESISTANCE; EMAMECTIN BENZOATE; ATLANTIC SALMON; IVERMECTIN RESISTANCE; REDUCED SENSITIVITY; DRUG-RESISTANCE; ABC;
D O I
10.1016/j.cbpc.2011.08.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The salmon louse, Lepeophtheirus salmonis, is a crustacean ectoparasite of salmonid fish. At present, sea louse control on salmon farms relies heavily upon chemical treatments. Drug efflux transport, mediated by ABC transporters such as P-glycoprotein (Pgp), represents a major mechanism for drug resistance in parasites. We report here the molecular cloning of a new Pgp from the salmon louse, called SL-PGY1. A partial Pgp sequence was obtained by searching sea louse ESTs, and extended by rapid amplification of cDNA ends (RACE). The open reading frame of SL-PGY1 encodes a protein of 1438 amino acids that possesses typical structural traits of P-glycoproteins, and shows a high degree of sequence homology to invertebrate and vertebrate P-glycoproteins. In the absence of drug exposure. SL-PGY1 mRNA expression levels did not differ between a drug-susceptible strain of L salmonis and a strain showing a similar to 7-fold decrease in sensitivity against emamectin benzoate, the active component of the in-feed sea louse treatment SLICE (R) (Merck Animal Health). Aqueous exposure of the hyposensitive salmon louse strain to emamectin benzoate (24 h, 410 mu g/L) provoked a 2.9-fold upregulation of SL-PGY1. Adult male lice of both strains showed a greater abundance of SL-PGY1 mRNA than adult females. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:198 / 205
页数:8
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