Micropatterned Hydrogels with Highly Ordered Cellulose Nanocrystals for Visually Monitoring Cardiomyocytes

被引:23
|
作者
Wang, Junmei [1 ]
Liu, Qian [2 ]
Gong, Jixing [2 ]
Wan, Zhongjun [2 ]
Zhou, Jinping [1 ]
Chang, Chunyu [1 ]
Zhang, Donghui [2 ]
机构
[1] Wuhan Univ, Coll Chem & Mol Sci, Hubei Engn Ctr Nat Polymer Based Med Mat, Wuhan 430072, Peoples R China
[2] Hubei Univ, Natl & Local Joint Engn Res Ctr High Throughput D, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Pr, Wuhan 430062, Peoples R China
基金
中国国家自然科学基金;
关键词
cardiomyocytes; highly ordered tunicate cellulose nanocrystals; micropatterned hydrogels; multi-chamber devices; visualized monitoring systems; CARDIAC MICROPHYSIOLOGICAL DEVICES; ORGANIZATION; HEART;
D O I
10.1002/smll.202202235
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Cardiac microphysiological systems are accurate in vitro platforms that reveal the biological mechanisms underlying cardiopathy, accelerating pharmaceutical research in this field. Current cardiac microphysiological devices and organs-on-chips consist of several layers prepared with complex, multi-step processes. Incorporating inorganic photonic crystals may cause long-term biocompatibility issues. Herein, micropatterned hydrogels with anisotropic structural colors are prepared by locking shear-oriented tunicate cellulose nanocrystals (TCNCs) in hydrogel networks through in situ polymerization, allowing the visualization and monitoring of cardiomyocytes. The anisotropic hydrogels are composed of highly ordered TCNCs with bright interference color and micro-grooved methacrylated gelatin with excellent biocompatibility. The microgroove patterns induce cardiomyocyte alignment and the autonomous beating of cardiomyocytes causes the hydrogels to deform, dynamically shifting the interference color. These micropatterned hydrogels could noninvasively monitor real-time changes of cardiomyocytes under pharmaceutical treatment and electrical stimulation through wavelength shifts in the transmittance spectra. This system provides a new way to detect the beat rate of cardiac tissue and it may contribute to high throughput develop.
引用
收藏
页数:9
相关论文
共 50 条
  • [41] Electro-osmotic Actuators from Cellulose Nanocrystals and Nanocomposite Hydrogels
    Reid, Lacey
    Hamad, Wadood Y.
    ACS APPLIED POLYMER MATERIALS, 2022, 4 (01) : 598 - 606
  • [42] Effect of carboxylated cellulose nanocrystals on konjac glucomannan/ κ-carrageenan composite hydrogels
    Tong, Cailing
    Huang, Chen
    Lai, Haoyu
    Li, Kailin
    Zeng, Xinxin
    Wen, Chengrong
    Wu, Chunhua
    Pang, Jie
    FOOD HYDROCOLLOIDS, 2024, 154
  • [43] Suspensions and hydrogels of cellulose nanocrystals (CNCs): characterization using microscopy and rheology
    Aref Abbasi Moud
    Milad Kamkar
    Amir Sanati-Nezhad
    Seyed Hossein Hejazi
    Cellulose, 2022, 29 : 3621 - 3653
  • [44] Designing pH-responsive and dielectric hydrogels from cellulose nanocrystals
    Gao, Xiaoyuan
    Sadasivuni, Kishor Kumar
    Kim, Hyun-Chan
    Min, Seung-Ki
    Kim, Jaehwan
    JOURNAL OF CHEMICAL SCIENCES, 2015, 127 (06) : 1119 - 1125
  • [45] Study of Cellulose Nanocrystals and Zinc Nitrate Hexahydrate Addition in Chitosan Hydrogels
    Ferreira, Sheila de Oliveira
    do Amaral Montanheiro, Thais Larissa
    Montagna, Larissa Stieven
    Guerrini, Lilia Mueller
    Lemes, Ana Paula
    MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, 2019, 22
  • [46] Suspensions and hydrogels of cellulose nanocrystals (CNCs): characterization using microscopy and rheology
    Moud, Aref Abbasi
    Kamkar, Milad
    Sanati-Nezhad, Amir
    Hejazi, Seyed Hossein
    CELLULOSE, 2022, 29 (07) : 3621 - 3653
  • [47] Highly Charged Cellulose Nanocrystals via Electrochemical Oxidation
    Yousefi, Neptun
    Hannonen, Jenna
    Fliri, Lukas
    Peljo, Pekka
    Kontturi, Eero
    NANO LETTERS, 2024, 24 (46) : 14610 - 14614
  • [48] Tough and highly stretchable polyacrylamide nanocomposite hydrogels with chitin nanocrystals
    Liu, Mingxian
    Huang, Jiandong
    Luo, Binghong
    Zhou, Changren
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 78 : 23 - 31
  • [49] Monodisperse highly ordered chitosan/cellulose nanocomposite foams
    Andrieux, Sebastien
    Medina, Lilian
    Herbst, Michael
    Berglund, Lars A.
    Stubenrauch, Cosima
    COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING, 2019, 125
  • [50] Micropatterned nanocellulose films and magnetically responsive cellulose nanocrystals as platforms for microfluidic devices and selective protein separation
    Guo, Jiaqi
    Filpponen, Erkko
    Johansson, Leena-Sisko
    Linder, Markus
    Ras, Robin
    Levkin, Pavel
    Rojas, Orlando
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 257