Self-assembled multilayer superstructures as immobilization support for bioreceptors

A new strategy for the preparation of layer-by-layer inorganic assemblies are discussed which allows simple fabrication of multifunctional thin film on various substrates. The procedure involves alternating layers of the cyanoferrate derivatives with layers of copper ions. A film growth was investigated in situ by surface plasmon resonance (SPR) and characterized by atomic force microscopy (AFM). It was shown that the topography of the surface strongly depends on a number of layers. In particular, the change-over to the formation of 3D aggregates after 12-15 cycles of deposition was observed. It was demonstrated that biological captors, immunoglobulins, can easily be immobilized onto these films in closely packed oriented monolayer, and stable theoretically predicted response was obtained in SPR geometry. This suggests that the functional multilayer built using this approach will have useful application owing to their controllable chemical reactivity. (C) 2003 Elsevier B.V. All rights reserved.