Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens

被引：17

作者：

Alonso, Maria ^{[1
,2
]}

Herranz, Marta ^{[1
,2
]}

Martinez Lirola, Miguel ^{[3
]}

Gonzalez-Rivera, Milagros ^{[2
,4
]}

Bouza, Emilio ^{[1
,2
]}

Garcia de Viedma, Dario ^{[1
,2
]}

机构：

[1] Hosp Gen Univ Gregorio Maranon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain

[2] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain

[3] Complejo Hosp Torrecardenas, Almeria, Spain

[4] Hosp Gregorio Maranon, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期

关键词：

MYCOBACTERIUM-TUBERCULOSIS;

D O I：

10.1128/JCM.00132-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.

引用

页码：1755 / 1757

页数：3

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