Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens

被引：17
|
作者：
Alonso, Maria [1 ,2 ]
Herranz, Marta [1 ,2 ]
Martinez Lirola, Miguel [3 ]
Gonzalez-Rivera, Milagros [2 ,4 ]
Bouza, Emilio [1 ,2 ]
Garcia de Viedma, Dario [1 ,2 ]
机构：
[1] Hosp Gen Univ Gregorio Maranon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain
[2] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
[3] Complejo Hosp Torrecardenas, Almeria, Spain
[4] Hosp Gregorio Maranon, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期
关键词：
MYCOBACTERIUM-TUBERCULOSIS;
D O I：
10.1128/JCM.00132-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
引用
收藏
页码：1755 / 1757
页数：3
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