Rapid detection of lineage IV peste des petits ruminants virus by real-time RT-PCR

被引：6

作者：

Li, Lin ^{[1
]}

Wu, Xiaodong ^{[1
]}

Liu, Fuxiao ^{[1
]}

Wang, Zhiliang ^{[1
]}

Liu, Chunju ^{[1
]}

Wang, Qinghua ^{[1
]}

Bao, Jingyue ^{[1
]}

机构：

[1] China Anim Hlth & Epidemiol Ctr, Natl Res Ctr Exot Anim Dis, 369 Nanjing Rd, Qingdao 266032, Shandong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2016年 / 235卷

关键词：

Peste des petits ruminants virus; Lineage IV; Differentiatial diagnosis; Real-time RT-PCR;

D O I：

10.1016/j.jviromet.2016.05.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peste des petits ruminants virus (PPRV) is the cause agent of peste des petitis ruminants (PPR). A novel lineage IV PPRV has reemerged in China in 2013 and 2014. Mass vaccination was implemented in most provinces in China. In order to detect lineage IV PPRV in clinical samples and to distinguish rapidly it from the other lineages PPRVs, a real-time RT-PCR assay was developed. This assay showed high sensitivity, specificity and efficiency in differentiating the lineage IV PPRV from others. The performance of this assay was evaluated by positive clinical samples of lineage IV viruses. This new real-time RT-PCR assay will facilitate epidemiological investigations and rapid differentiatial diagnosis in areas where lineage IV viruses are circulating. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：131 / 133

页数：3

共 50 条

[41] Molecular Epidemiology of Peste Des Petits Ruminants Virus in West Africa: Is Lineage IV Replacing Lineage II in Burkina Faso?
Biguezoton, Abel S.
Ilboudo, Guy Sidwatta
Wieland, Barbara
Sawadogo, Rahinata W-Y.
Dah, Fairou F.
Sidibe, Cheick A. K.
Zoungrana, Adrien
Okoth, Edward
Dione, Michel
VIRUSES-BASEL, 2024, 16 (02):
[42] Peste des petits ruminants in Nigeria: identification and variations of lineage II and lineage IV in sheep and goats
Chukwudi, Ijeoma Chekwube
Mira, Francesco
Ogbu, Kenneth Ikejiofor
Malesa, Refiloe Petunia
Purpari, Giuseppa
Luka, Pam Dachung
Ugochukwu, Emmanuel Ikenna
Heath, Livio Edward
Guercio, Annalisa
Chah, Kennedy Foinkfu
VETERINARIA ITALIANA, 2021, 57 (03) : 189 - 199
[43] Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay
Couacy-Hymann, E
Roger, F
Hurard, C
Guillou, JP
Libeau, G
Diallo, A
JOURNAL OF VIROLOGICAL METHODS, 2002, 100 (1-2) : 17 - 25
[44] Development of recombinase polymerase amplification assays for the rapid detection of peste des petits ruminants virus
Zhang, Yongning
Wang, Jianchang
Zhang, Zhou
Mei, Lin
Wang, Jinfeng
Wu, Shaoqiang
Lin, Xiangmei
JOURNAL OF VIROLOGICAL METHODS, 2018, 254 : 35 - 39
[45] Comparative sensitivity of sandwich ELISA, RT-PCR, and real-time RT-PCR for detection of bluetongue virus
Chand, Karam
Biswas, S. K.
Mondal, B.
INDIAN JOURNAL OF ANIMAL SCIENCES, 2018, 88 (02): : 191 - 193
[46] Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR
Tan, Eng Lee
Yong, Li Li Gaynor
Quak, Seng Hock
Yeo, Wei Cheng Andrea
Chow, Vincent Tak Kwong
Poh, Chit Laa
JOURNAL OF CLINICAL VIROLOGY, 2008, 42 (02) : 203 - 206
[47] Rapid detection of infectious adenoviruses by mRNA real-time RT-PCR
Ko, G
Jothikumar, N
Hill, VR
Sobsey, MD
JOURNAL OF VIROLOGICAL METHODS, 2005, 127 (02) : 148 - 153
[48] First Complete Genome Sequence of a Lineage III Peste des Petits Ruminants Virus
Dundon, William G.
Kihu, Simon M.
Settypalli, T. Bharani K.
Gitao, George C.
Bebora, Lily C.
John, Njenga M.
Oyugi, Julius O.
Silber, Roland
Loitsch, Angelika
Diallo, Adama
GENOME ANNOUNCEMENTS, 2014, 2 (05)
[49] Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR
Tewari, Deepanker
Zellers, Corey
Acland, Helen
Pedersen, Janice C.
JOURNAL OF CLINICAL VIROLOGY, 2007, 40 (02) : 142 - 145
[50] Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR
Todd, Angela K.
Costa, Anna-Maria
Waller, Gregory
Daley, Andrew J.
Barr, Ian G.
Deng, Yi-Mo
JOURNAL OF VIROLOGICAL METHODS, 2021, 294

← 1 2 3 4 5 →