MOLECULAR CHARACTERIZATION OF ARACHIS HYPOGAEA NAC 2 (AHNAC2) REVEALS IT AS A NAC-LIKE PROTEIN IN PEANUT

被引：8
|
作者：
Liu, X. [1 ]
Zhang, B. Y. [1 ]
Hong, L. [1 ]
Su, L. C. [1 ]
Liang, X. Q. [2 ]
Li, X. Y. [3 ]
Li, L. [1 ]
机构：
[1] S China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou, Guangdong, Peoples R China
[2] Guangdong Acad Agr Sci, Cross Res Inst, Guangzhou, Guangdong, Peoples R China
[3] So Med Univ, Inst Genet Engn, Guangzhou, Guangdong, Peoples R China
来源：
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2010年 / 24卷 / 04期
关键词：
NAC domain; localization; transcriptional activity; peanut; TRANSCRIPTION FACTORS; ARABIDOPSIS; GENES;
D O I：
10.2478/V10133-010-0085-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The NAC (NAM ATAF and CUC) proteins containing the consensus NAC domain are plant specific transcriptional regulators and are expressed in various developmental stages and tissues We have previously reported the isolation and expression pattern of a peanut NAC gene AhNAC2 (Arachis hypogaea NAC2) In the present study it is indicated that AhNAC2-GFP fusion protein was localized in the peanut protoplast nucleus and the C terminal of AhNAC2-GAL4BD fusion protein showed transcriptional activity in yeast The AhNAC2-His protein could bind to specific NACRE (NAC Response Elements) in vitro Thus the results presented here contribute to our understanding of the specificity and molecular functions of AhNAC2 protein acting as transcriptional factor
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收藏
页码：2066 / 2070
页数：5
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