Design of modular dual enzyme-responsive peptides

被引：2
|
作者：
Boehnke, Natalie
Maynard, Heather D. [1 ,2 ]
机构：
[1] Univ Calif Los Angeles, Dept Chem & Biochem, 607 Charles E Young Dr East, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Calif NanoSyst Inst, 607 Charles E Young Dr East, Los Angeles, CA 90095 USA
来源：
BIOPOLYMERS | 2017年 / 108卷 / 05期
关键词：
dual-enzyme; dual-enzyme assay; indirect enzyme detection; multi-enzyme; multi-enzyme assay; nitroanilide; CANCER; CASPASE-3; HYDROLYSIS; PROBE; SPECIFICITY; DELIVERY; ARGININE; CELLS; ASSAY;
D O I：
10.1002/bip.23035
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Dual enzyme-responsive peptides were synthesized by masking the e-amine of lysine with various enzyme substrates. Enzymatic cleavage of these sequences unmasked the e-amine, allowing for further digestion by a second enzyme, which was monitored colorimetrically. This modular peptide design should provide substrates for a large combination of clinically relevant enzymes.
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页数：6
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