Simultaneous Determination of Methotrexate and Sulphasalazine in Plasma by HPLC-DAD

被引：0
|
作者：
Joseph, Siji [1 ]
Menon, Sreelakshmy [1 ]
Khera, Smriti [2 ]
机构：
[1] Agilent Technol India Pvt Ltd, Life Sci Ctr, Bangalore, Karnataka, India
[2] Agilent Technol, Life Sci & Diagnost Grp, Santa Clara, CA 95051 USA
来源：
LC GC EUROPE | 2015年 / 28卷 / 05期
关键词：
RHEUMATOID-ARTHRITIS; COMBINATION THERAPY; DOUBLE-BLIND; EFFICACY;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Here we describe a simple and robust high performance liquid chromatography-diode array detection (HPLC-DAD) method for the simultaneous determination of methotrexate (MTX) and sulphasalazine (SSZ) from plasma. MTX and SSZ are used in combination for the treatment of rheumatoid arthritis. Using two detector wavelengths, 304 nm for MTX and 358 nm for SSZ, we were able to selectively quantitate both analytes during the same chromatographic run. The method was validated using quality control samples for critical analytical performance criteria of recovery, reproducibility, selectivity, accuracy, and precision.
引用
收藏
页码：272 / +
页数：5
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