Isolation and characterization of polymorphic microsatellite loci in Asiatic sand sedge, Carex kobomugi Ohwi (Cyperaceae)

被引：15
|
作者：
Ohsako, Takanori
Yamane, Kyoko
机构：
[1] Kyoto Prefectural Univ, Grad Sch Agr, Lab Agroecol, Kyoto 6190244, Japan
[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Plant Resource Lab, Naka Ku, Osaka 5998531, Japan
来源：
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期
关键词：
Asiatic sand sedge; Carex kobomugi; clonal propagation; coastal plant; microsatellite loci;
D O I：
10.1111/j.1471-8286.2007.01761.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fourteen polymorphic microsatellite loci were isolated from Asiatic sand sedge, Carex kobomugi Ohwi (Cyperaceae), a clonal perennial herb predominant in sandy coast vegetation in Japan. Polymorphism was surveyed for 24 ramets within a single population and for eight distinct individuals from six populations. Within a population, between two and eight alleles were found per locus, and expected heterozygosity ranged from 0.041 to 0.720. Among populations, a higher level of polymorphism was observed. The microsatellite loci were shown to be useful for the study of spatial genetic structure at fine scale and genetic differentiation among populations.
引用
收藏
页码：1023 / 1025
页数：3
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