Quantitative imaging and measurement of cell-substrate surface deformation by digital holography

被引：15
|
作者：
Yu, Xiao [1 ]
Cross, Michael [2 ]
Liu, Changgeng [1 ]
Clark, David C. [1 ]
Haynie, Donald T. [2 ]
Kim, Myung K. [1 ]
机构：
[1] Univ S Florida, Dept Phys, Digital Holog & Microscopy Lab, Tampa, FL 33620 USA
[2] Univ S Florida, Dept Phys, Nanomed & Nanobiotechnol Lab, Tampa, FL 33620 USA
来源：
JOURNAL OF MODERN OPTICS | 2012年 / 59卷 / 18期
关键词：
digital holography; quantitative phase microscopy; cell-substrate deformation; traction force; TOTAL INTERNAL-REFLECTION; TRACTION FIELDS; LOCOMOTION; FORCE; MICROSCOPY; FIBROBLASTS; MODULI; LAYERS; FEEL;
D O I：
10.1080/09500340.2012.729095
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Quantitative phase microscopy by digital holography (DH-QPM) is introduced to study the cell-substrate interactions and migratory behavior of adhesive cells. A non-wrinkling elastic substrate, collagen-coated polyacrylamide (PAA) has been employed and its surface deformation due to cell adhesion and motility has been visualized as certain tangential and vertical displacement and distortion. The surface deformation on substrates of different elasticity and thickness has been quantitatively imaged and the corresponding cellular traction force of motile fibroblasts has been measured from phase profiles by DH-QPM. DH-QPM is able to yield quantitative measures directly and provide efficient and versatile means for quantitatively analyzing cellular motility.
引用
收藏
页码：1591 / 1598
页数：8
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