DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4

被引:81
|
作者
Tanaka, Tetsuya [3 ]
Legat, Amandine [4 ]
Adam, Emmanuelle [3 ]
Steuve, Jonathan [3 ]
Gatot, Jean-Stephane [3 ]
Vandenbranden, Michel [4 ]
Ulianov, Liliana [4 ]
Lonez, Caroline [4 ]
Ruysschaert, Jean-Marie [4 ]
Muraille, Eric [5 ]
Tuynder, Marcel [2 ]
Goldman, Michel [1 ,2 ]
Jacquet, Alain [3 ]
机构
[1] Univ Libre Bruxelles, Inst Med Immunol, B-6041 Charleroi, Belgium
[2] Univ Libre Bruxelles, Inst Med Immunol, B-6041 Gosselies, Belgium
[3] Univ Libre Bruxelles, Inst Biol & Med Mol, Lab Allergol Expt, B-6041 Charleroi, Belgium
[4] Univ Libre Bruxelles, Lab Struct & Fonct Membranes Biol, Brussels, Belgium
[5] Univ Libre Bruxelles, Parasitol Lab, Brussels, Belgium
关键词
adjuvants; cationic liposome; dendritic cells; Toll-like receptors;
D O I
10.1002/eji.200737998
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
DiC14-amidine cationic liposomes were recently shown to promote Th1 responses when mixed with allergen. To further define the mode of action of diC14-amidine as potential vaccine adjuvant, we characterized its effects on mouse and human myeloid dendritic cells (DC). First, we observed that, as compared with two other cationic liposomes, only diC14-amidine liposomes induced the production of IL-12p40 and TNF-alpha by mouse bone marrow-derived DC. DiC14-amidine liposomes also activated human DC, as shown by synthesis of IL-12p4O and TNF-alpha, accumulation of IL-6, IFN-beta and CXCL10 mRNA, and up-regulation of membrane expression of CD80 and CD86. DC stimulation by diC14-amidine liposomes was associated with activation of NF-kappa B, ERK1/2, JNK and p38 MAP kinases. Finally, we demonstrated in mouse and human cells that diC14-amidine liposomes use Toll-like receptor 4 to elicit both MyD88-dependent and Toll/IL-IR-containing adaptor inducing interferon IFN-beta (TRIF)-dependent responses.
引用
收藏
页码:1351 / 1357
页数:7
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