Discovery of a New Pyrazinone Natural Product by Genome Mining

被引：0
|
作者：
Kang, Wen-Jia [1 ]
Wu, Sheng [2 ]
Hua, Huiming [1 ]
Pan, Hai-Xue [1 ]
Tang, Gong-Li [2 ]
机构：
[1] Shenyang Pharmaceut Univ, Minist Educ, Key Lab Struct Based Drug Design & Discovery, Shenyang 110016, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China
来源：
CHINESE JOURNAL OF ORGANIC CHEMISTRY | 2016年 / 36卷 / 07期
基金：
中国国家自然科学基金;
关键词：
pyrazinone; Streptomyces; genome mining; biosynthesis;
D O I：
10.6023/cjoc201602016
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The genomic sequences of Streptomyces sp. TP-A0365 were scanned using a secondary metabolite genome mining approach. A new nonribosomal peptide synthetase (NRPS) gene responsible for the biosynthesis of an unknown pyrazinone compound was found. The biosynthetic pathway of related compound was disrupted by inactivating the NRPS gene. Comparing the fermentation products of the mutant strain and the original strain (TG1301 derived from Streptomyces sp. TP-A0365), we caught compound 1 which disappeared in the mutant strain. Compound 1 was isolated from 20 L fermentation cultures of the original strain and its structure was established as 3-isopropyl-7,8-dihydropyrrolo[1,2-a]pyrazin-4(6H)-one, a new pyrazinone compound designated as provalin
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收藏
页码：1696 / 1699
页数：4
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