Micronucleus test of diphenylchloroarsine in mice

The micronucleus test in mice's marrow (myeloid) cells was used to research the effect of diphenylchloroarsine (DA) on inducing chromosome damage of polychromatophilic erythrocyte. Through pre-experiment, DAs LD50 in mice (ig) is determined, which is 28.07 mg/kg, and the time of gaining marrow cells is in exposure 24 h. Five groups have been determined in this test: solvent control (sesame), positive control (Cyclophosphamide: 100 mg/kg), low-dose group (1/8 LD50: 3.51 mg/kg), mid-dose group (1/4 LD50: 7.02 mg/kg), high-dose group (1/2 LD50: 14.03mg/kg). 6 male mice are in each group. The result indicates the values of polychromatophilic erythrocyte to normochromatophilic erythrocyte (PCE/NCE) of those groups are 2.44, 1.81, 1.99, 1.90 and 2.04 respective, the relative ratios of micronucleus are 2.00parts per thousand, 79.67parts per thousand, 2.17parts per thousand,1.17parts per thousand, 1.67parts per thousand. There are no differences between solvent control and any DA dose group (p>0.05). And there is extremely significant difference between solvent control and positive control (p<0.01). Therefore, the effects of DA on mice marrow micronucleus rates are negative in this test condition.