Structure of the male determinant factor for Brassica self-incompatibility

被引:42
|
作者
Mishima, M
Takayama, S
Sasaki, K
Jee, J
Kojima, C
Isogai, A
Shirakawa, M
机构
[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma 6300101, Japan
[2] Yokohama City Univ, Grad Sch Integrated Sci, Div Mol Biophys, Yokohama, Kanagawa 2300045, Japan
[3] RIKEN, Genom Sci Ctr, Kanagawa 2300045, Japan
关键词
D O I
10.1074/jbc.M305305200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/ non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S-8-haplotype ( S-8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hypervariable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor.
引用
收藏
页码:36389 / 36395
页数:7
相关论文
共 50 条
  • [21] Molecular mechanism of self-incompatibility Brassica.
    Shimosato, H
    Takayama, S
    Shiba, H
    Fang-Sik, C
    Iwano, M
    Isogai, A
    PLANT AND CELL PHYSIOLOGY, 2002, 43 : S34 - S34
  • [22] DRY STIGMAS, WATER AND SELF-INCOMPATIBILITY IN BRASSICA
    DICKINSON, H
    SEXUAL PLANT REPRODUCTION, 1995, 8 (01): : 1 - 10
  • [23] A receptor kinase and the self-incompatibility response in Brassica
    Cock, JM
    ADVANCES IN BOTANICAL RESEARCH INCORPORATING ADVANCES IN PLANT PATHOLOGY, VOL 32: PLANT PROTEIN KINASES, 2000, 32 : 269 - 298
  • [24] IMMUNOGENETICS OF SELF-INCOMPATIBILITY IN BRASSICA OLERACEA L
    NASRALLAH, ME
    WALLACE, DH
    HEREDITY, 1967, 22 : 519 - +
  • [25] MOLECULAR-GENETICS OF SELF-INCOMPATIBILITY IN BRASSICA
    NASRALLAH, JB
    PLANT REPRODUCTION : FROM FLORAL INDUCTION TO POLLINATION, 1989, 1 : 156 - 164
  • [26] Molecular mechanism of self-incompatibility in Brassica species
    Watanabe, M
    Kakizaki, T
    Takada, Y
    Suzuki, G
    Shiba, H
    Takayama, S
    Isogai, A
    PLANT AND CELL PHYSIOLOGY, 2003, 44 : S20 - S20
  • [27] Immunohistochemical studies on translocation of pollen S-haplotype determinant in self-incompatibility of Brassica rapa
    Iwano, M
    Shiba, H
    Funato, M
    Shimosato, H
    Takayama, S
    Isogai, A
    PLANT AND CELL PHYSIOLOGY, 2003, 44 (04) : 428 - 436
  • [28] The Identification and Analysis of the Self-Incompatibility Pollen Determinant Factor SLF in Lycium barbarum
    Wu, Jiali
    Nan, Xiongxiong
    Zhang, Xin
    Xu, Wendi
    Ma, Haijun
    Yang, Zijun
    Wang, Cuiping
    PLANTS-BASEL, 2024, 13 (07):
  • [29] Resynthesis of Brassica napus L. for self-incompatibility:: self-incompatibility reaction, inheritance and breeding potential
    Rahman, MH
    PLANT BREEDING, 2005, 124 (01) : 13 - 19
  • [30] Molecular mechanisms of self-recognition in Brassica self-incompatibility
    Brugière, N
    Cui, YH
    Rothstein, SJ
    TRENDS IN PLANT SCIENCE, 2000, 5 (10) : 432 - 438