Development of a Direct and Rapid Detection Method for Viable but Non-culturable State of Pediococcus acidilactici

被引：7

作者：

Guan, Yu ^{[1
]}

Wang, Kan ^{[2
]}

Zeng, Yang ^{[3
]}

Ye, Yanrui ^{[4
]}

Chen, Ling ^{[5
]}

Huang, Tengyi ^{[6
]}

机构：

[1] Capital Med Univ, Beijing Friendship Hosp, Dept Urol, Beijing, Peoples R China

[2] Shantou Univ Med Coll, Affiliated Hosp 2, Ctr Translat Med, Shantou, Peoples R China

[3] Shantou Univ Med Coll, Shantou, Peoples R China

[4] South China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Peoples R China

[5] South China Univ Technol, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou, Peoples R China

[6] Shantou Univ Med Coll, Affiliated Hosp 2, Dept Lab Med, Shantou, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷

关键词：

Pediococcus acidilactici; viable but non-culturable; artificially contaminated food; PMA-CPA; rapid detection; safety control; STAPHYLOCOCCUS-AUREUS BIOFILM; PATHOGENIC FEATURES; SOUTHERN CHINA; VIBRIO-PARAHAEMOLYTICUS; CURRENT METHODOLOGIES; BACTERICIDAL ACTIVITY; E; COLI; INTEGRON; AMPLIFICATION; SURVEILLANCE;

D O I：

10.3389/fmicb.2021.687691

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pediococcus acidilactici may significantly reduce the pH-value, and thus has different influence, including serving as a probiotic in human microbiota but a spoilage in human food as it could change the flavor. Pediococcus acidilactici is also capable of entering into the viable but non-culturable (VBNC) state causing false negative results of standard culture-based detection method. Thus, development of detection method for VBNC state P. acidilactici is of great significance. In this study, propidium monoazide (PMA) combined with cross priming amplification (CPA) was developed to detect the VBNC cells of P. acidilactici and applied on the detection in different systems. With detection limit of 10(4) cells/ml, high sensitivity, and 100% specificity, PMA-CPA can successfully detect VBNC cells of P. acidilactici and be applied in with high robustness.

引用

页数：7

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