Development of a Direct and Rapid Detection Method for Viable but Non-culturable State of Pediococcus acidilactici

被引：7
|
作者：
Guan, Yu [1 ]
Wang, Kan [2 ]
Zeng, Yang [3 ]
Ye, Yanrui [4 ]
Chen, Ling [5 ]
Huang, Tengyi [6 ]
机构：
[1] Capital Med Univ, Beijing Friendship Hosp, Dept Urol, Beijing, Peoples R China
[2] Shantou Univ Med Coll, Affiliated Hosp 2, Ctr Translat Med, Shantou, Peoples R China
[3] Shantou Univ Med Coll, Shantou, Peoples R China
[4] South China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Peoples R China
[5] South China Univ Technol, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou, Peoples R China
[6] Shantou Univ Med Coll, Affiliated Hosp 2, Dept Lab Med, Shantou, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷
关键词：
Pediococcus acidilactici; viable but non-culturable; artificially contaminated food; PMA-CPA; rapid detection; safety control; STAPHYLOCOCCUS-AUREUS BIOFILM; PATHOGENIC FEATURES; SOUTHERN CHINA; VIBRIO-PARAHAEMOLYTICUS; CURRENT METHODOLOGIES; BACTERICIDAL ACTIVITY; E; COLI; INTEGRON; AMPLIFICATION; SURVEILLANCE;
D O I：
10.3389/fmicb.2021.687691
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Pediococcus acidilactici may significantly reduce the pH-value, and thus has different influence, including serving as a probiotic in human microbiota but a spoilage in human food as it could change the flavor. Pediococcus acidilactici is also capable of entering into the viable but non-culturable (VBNC) state causing false negative results of standard culture-based detection method. Thus, development of detection method for VBNC state P. acidilactici is of great significance. In this study, propidium monoazide (PMA) combined with cross priming amplification (CPA) was developed to detect the VBNC cells of P. acidilactici and applied on the detection in different systems. With detection limit of 10(4) cells/ml, high sensitivity, and 100% specificity, PMA-CPA can successfully detect VBNC cells of P. acidilactici and be applied in with high robustness.
引用
收藏
页数：7
相关论文
共 50 条
  • [1] Induction, detection, formation, and resuscitation of viable but non-culturable state microorganisms
    Dong, Kai
    Pan, Hanxu
    Yang, Dong
    Rao, Lei
    Zhao, Liang
    Wang, Yongtao
    Liao, Xiaojun
    COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2020, 19 (01) : 149 - 183
  • [2] Development of a rapid method to detect and to monitor bacteriophage amplification in contact with viable but non-culturable bacteria
    Said, Myriam Ben
    Bousselmi, Latifa
    Ghrabi, Ahmed
    ENVIRONMENTAL TECHNOLOGY, 2024, 45 (16) : 3244 - 3252
  • [3] Detection and Quantification of Viable but Non-culturable Campylobacter jejuni
    Lv, Ruiling
    Wang, Kaidi
    Feng, Jinsong
    Heeney, Dustin D.
    Liu, Donghong
    Lu, Xiaonan
    FRONTIERS IN MICROBIOLOGY, 2020, 10
  • [4] PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state
    Yan, Muxia
    Xu, Ling
    Jiang, Hua
    Zhou, Zhenwen
    Zhou, Shishui
    Zhang, Li
    MICROBIAL PATHOGENESIS, 2017, 105 : 245 - 250
  • [5] The importance of the viable but non-culturable state in human bacterial pathogens
    Li, Laam
    Mendis, Nilmini
    Trigui, Hana
    Oliver, James D.
    Faucher, Sebastien P.
    FRONTIERS IN MICROBIOLOGY, 2014, 5 : 1 - 1
  • [6] The viable but non-culturable state and its relevance in food safety
    Ayrapetyan, Mesrop
    Oliver, James D.
    CURRENT OPINION IN FOOD SCIENCE, 2016, 8 : 127 - 133
  • [7] Viable but non-culturable form of bacteria
    Oezcakir, Olcay
    MIKROBIYOLOJI BULTENI, 2007, 41 (03): : 477 - 484
  • [8] 'Viable but non-culturable cells' are dead
    Song, Sooyeon
    Wood, Thomas K.
    ENVIRONMENTAL MICROBIOLOGY, 2021, 23 (05) : 2335 - 2338
  • [9] Roles of viable but non-culturable state in the survival of Campylobacter jejuni
    Santos, Leticia Silva
    Rossi, Daise Aparecida
    Braz, Raquelline Figueiredo
    Fonseca, Belchiolina Beatriz
    Guidotti-Takeuchi, Micaela
    Alves, Rosiane Nascimento
    Beletti, Marcelo Emilio
    Almeida-Souza, Hebreia Oliveira
    Maia, Larissa Prado
    Santos, Paula de Souza
    de Souza, Jessica Brito
    de Melo, Roberta Torres
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2023, 13
  • [10] Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens
    Zhao, Xihong
    Zhong, Junliang
    Wei, Caijiao
    Lin, Chii-Wann
    Ding, Tian
    FRONTIERS IN MICROBIOLOGY, 2017, 8
12345