Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue

被引：42
|
作者：
Sidamonidze, Ketevan [1 ]
Peck, Marcia K. [1 ]
Perez, Michael [1 ]
Baumgardner, Dennis [2 ]
Smith, Gerald [3 ]
Chaturvedi, Vishnu [1 ,4 ]
Chaturvedi, Sudha [1 ,4 ]
机构：
[1] New York State Dept Hlth, Wadsworth Ctr, Mycol Lab, Albany, NY USA
[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Family Med, Milwaukee, WI 53201 USA
[3] Aurora Hlth Care, Dept Pathol, Milwaukee, WI USA
[4] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期
关键词：
GENETIC DIVERSITY; DNA; EPIDEMIOLOGY; SAMPLES;
D O I：
10.1128/JCM.00310-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
引用
收藏
页码：1783 / 1786
页数：4
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