Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue

被引：42

作者：

Sidamonidze, Ketevan ^{[1
]}

Peck, Marcia K. ^{[1
]}

Perez, Michael ^{[1
]}

Baumgardner, Dennis ^{[2
]}

Smith, Gerald ^{[3
]}

Chaturvedi, Vishnu ^{[1
,4
]}

Chaturvedi, Sudha ^{[1
,4
]}

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Mycol Lab, Albany, NY USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Family Med, Milwaukee, WI 53201 USA

[3] Aurora Hlth Care, Dept Pathol, Milwaukee, WI USA

[4] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期

关键词：

GENETIC DIVERSITY; DNA; EPIDEMIOLOGY; SAMPLES;

D O I：

10.1128/JCM.00310-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.

引用

页码：1783 / 1786

页数：4

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