Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides

被引：2
|
作者：
Fauconnier, ML
Marlier, M
机构：
[1] U.E. R. de Chim. Gen. et Organique, Fac. Univ. des Sciences Agronomiques, B-5030 Gembloux, Passage des Deportes
来源：
GRASAS Y ACEITES | 1996年 / 47卷 / 04期
关键词：
hydroperoxide; linoleic acid; linolenic acid; lipoxygenase; soybean;
D O I：
10.3989/gya.1996.v47.i4.865
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Purification of soybean lipoxygenase isoenzyme-l was reinvestigated furnishing a new procedure. Different parameters influencing the hydroperoxide production were studied. Our study focused particularly on the enzyme activity inhibition by the reaction products. Dixon plots revealed competitive inhibition with Ki value of 0.45 and 1.5 mM respectively for 13-linoleic and 13-linolenic acid hydroperoxides.
引用
收藏
页码：242 / 246
页数：5
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