Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides

被引：2
|
作者：
Fauconnier, ML
Marlier, M
机构：
[1] U.E. R. de Chim. Gen. et Organique, Fac. Univ. des Sciences Agronomiques, B-5030 Gembloux, Passage des Deportes
来源：
GRASAS Y ACEITES | 1996年 / 47卷 / 04期
关键词：
hydroperoxide; linoleic acid; linolenic acid; lipoxygenase; soybean;
D O I：
10.3989/gya.1996.v47.i4.865
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Purification of soybean lipoxygenase isoenzyme-l was reinvestigated furnishing a new procedure. Different parameters influencing the hydroperoxide production were studied. Our study focused particularly on the enzyme activity inhibition by the reaction products. Dixon plots revealed competitive inhibition with Ki value of 0.45 and 1.5 mM respectively for 13-linoleic and 13-linolenic acid hydroperoxides.
引用
收藏
页码：242 / 246
页数：5
相关论文
共 50 条
  • [1] PURIFICATION AND CHARACTERIZATION OF 2 FORMS OF HYDROGENASE ISOENZYME-1 FROM ESCHERICHIA-COLI
    FRANCIS, K
    PATEL, P
    WENDT, JC
    SHANMUGAM, KT
    JOURNAL OF BACTERIOLOGY, 1990, 172 (10) : 5750 - 5757
  • [2] Anaerobic lipoxygenase activity from Chlorella pyrenoidosa responsible for the cleavage of the 13-hydroperoxides of linoleic and linolenic acids
    Nuñez, A
    Foglia, TA
    Savary, BJ
    Piazza, GJ
    EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2000, 102 (03) : 181 - 188
  • [3] PURIFICATION OF ISOENZYME-1 OF PHOSPHOLIPASE-C FROM CLOSTRIDIUM-PERFRINGENS AND SOME OF ITS PROPERTIES
    RAKHIMOV, MM
    AKHMEDZHANOV, RA
    SOFINA, EY
    SAGATOVA, FA
    SHEMANOVA, GF
    BIOCHEMISTRY-MOSCOW, 1989, 54 (08) : 1081 - 1088
  • [4] PURIFICATION OF LACTATE-DEHYDROGENASE ISOENZYME-1, ISOENZYME-2, AND ISOENZYME-3 FROM HUMAN-ERYTHROCYTES
    PRIDGAR, EM
    MOSES, GC
    HENDERSON, AR
    CLINICAL CHEMISTRY, 1984, 30 (08) : 1353 - 1357
  • [5] LACTATE-DEHYDROGENASE ISOENZYME-1 DETERMINED BY INHIBITION WITH 1,6-HEXANEDIOL
    SHAMBERGER, RJ
    CLINICAL CHEMISTRY, 1987, 33 (06) : 992 - 992
  • [6] Structure of soybean lipoxygenase L3 and a comparison with its L1 isoenzyme
    SkrzypczakJankun, E
    Amzel, LM
    Kroa, BA
    Funk, MO
    PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1997, 29 (01) : 15 - 31
  • [7] Synthesis of Linoleic Acid 13-Hydroperoxides from Safflower Oil Utilizing Lipoxygenase in a Coupled Enzyme System with In-Situ Oxygen Generation
    Marti, Valentin Gala
    Coenen, Anna
    Schoerken, Ulrich
    CATALYSTS, 2021, 11 (09)
  • [8] PURIFICATION AND PROPERTIES OF A CALCIUM REQUIRING LIPOXYGENASE ISOENZYME FROM SOYBEAN (GLYCINUS-MAX) SEEDS
    DREESEN, TD
    KOCH, RB
    FEDERATION PROCEEDINGS, 1981, 40 (06) : 1670 - 1670
  • [9] LACTATE-DEHYDROGENASE ISOENZYME-1 IN SERUM DETERMINED BY SODIUM-PERCHLORATE INHIBITION
    PANTEGHINI, M
    BONORA, R
    PAGANI, F
    CLINICAL CHEMISTRY, 1991, 37 (07) : 1300 - 1300
  • [10] A novel method for isolation of lipoxygenase-1 isoenzyme from soybean meal
    Németh, A.Sz.
    Szajáni, B.
    Márczy, J.Sz.
    Simon, M.L.
    Hungarian Journal of Industrial Chemistry, 2001, 29 (01): : 35 - 38
12345