Molecular cloning of canine Wilms' tumor 1 for immunohistochemical analysis in canine tissues

被引：5
|
作者：
Sakai, Osamu [1 ]
Sakurai, Masashi [2 ]
Sakai, Hiroki [3 ]
Kubo, Masahito [2 ]
Hiraoka, Hiroko [1 ]
Baba, Kenji [4 ]
Okuda, Masaru [4 ]
Mizuno, Takuya [1 ]
机构：
[1] Yamaguchi Univ, United Grad Sch Vet Med, Lab Mol Diagnost & Therapeut, 1677-1 Yoshida, Yamaguchi 7538515, Japan
[2] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Pathol, Yamaguchi 7538515, Japan
[3] Gifu Univ, Lab Vet Pathol, Pathogenet Vet Med, Joint Dept Vet Med,Fac Appl Biol Sci, Gifu 5011193, Japan
[4] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Internal Med, Yamaguchi 7538515, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2017年 / 79卷 / 07期
关键词：
cancer; canine; immunohistochemical staining; Wilms' tumor 1; WT1 PEPTIDE VACCINE; GENE-PRODUCT; CANCER; PATIENT; CML;
D O I：
10.1292/jvms.17-0229
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Wilms' tumor 1 (WT1) expression has been investigated in various human cancers as a target molecule for cancer immunotherapy. However, few studies have focused on WT1 expression in dogs. Firstly, cDNA of canine WT1 (cWT1) was molecularly cloned from normal canine kidney. The cross-reactivity of the anti-human WT1 monoclonal antibody (6F-H2) with cWT1 was confirmed via Western blotting using cells overexpressing cWT1. Immunohistochemical staining revealed that cWT1 expression was detected in all canine lymphoma tissues and in some normal canine tissues, including the kidney and lymph node. cWT1 is a potential immunotherapy target against canine cancers.
引用
收藏
页码：1272 / 1277
页数：6
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