Antigenic mapping of cytochrome P450 101 (P450cam)

被引:0
|
作者
Kolesanova, EF [1 ]
Kozin, SA [1 ]
Archakov, AI [1 ]
机构
[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119832, Russia
来源
BIOORGANICHESKAYA KHIMIYA | 1998年 / 24卷 / 10期
关键词
cytochrome P450; antigenic determinants; peptide scanning;
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摘要
Eighteen linear antigenic sites were found in cytochrome P450 101 (P450cam) from Pseudomonas putida by the peptide scanning method. These sites accounted for about 30% of the protein sequence. We found no sequences that completely coincided with the antigenic sites of P450cam in cytochromes P450 from other sources. The linear B-epitopes of P540cam were mainly localized on the boundaries separating the elements of the secondary structure. Seventeen of eighteen antigenic sites were found-to be on the protein surface and accessible to water molecules. Many functionally important sites or amino acid residues of the P350cam molecule coincided or were in close proximity to the linear B-epitopes found.
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页码:747 / 755
页数:9
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