Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

被引：11
|
作者：
McConnell, G
Smith, GL
Girkin, JM
Gurney, AM
Ferguson, AI
机构：
[1] Univ Strathclyde, Ctr Biophoton, Glasgow G4 0NR, Lanark, Scotland
[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
[3] Univ Strathclyde, Inst Photon, Glasgow G4 0NW, Lanark, Scotland
[4] Univ Strathclyde, Dept Physiol & Pharmacol, Glasgow G4 0NR, Lanark, Scotland
[5] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland
来源：
OPTICS LETTERS | 2003年 / 28卷 / 19期
关键词：
D O I：
10.1364/OL.28.001742
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2. (C) 2003 Optical Society of America.
引用
收藏
页码：1742 / 1744
页数：3
相关论文
共 25 条
  • [21] Efficient all-solid-state tunable source based on a passively Q-switched high-power Nd:YAG laser
    A. Agnesi
    E. Piccinini
    G.C. Reali
    C. Solcia
    Applied Physics B, 1997, 65 : 303 - 305
  • [22] Efficient all-solid-state tunable source based on a passively Q-switched high-power Nd:YAG laser
    Agnesi, A
    Piccinini, E
    Reali, GC
    Solcia, C
    APPLIED PHYSICS B-LASERS AND OPTICS, 1997, 65 (02): : 303 - 305
  • [23] Two-photon excitation of 2,5-diphenyloxazole using a low power green solid state laser
    Luchowski, Rafal
    CHEMICAL PHYSICS LETTERS, 2011, 501 (4-6) : 572 - 574
  • [24] All-solid-state continuous-wave tunable blue-light source by intracavity doubling of a diode-pumped Cr:LiSAF laser
    1600, Optical Soc of America, Washington, DC, USA (20):
  • [25] ALL-SOLID-STATE CONTINUOUS-WAVE TUNABLE BLUE-LIGHT SOURCE BY INTRACAVITY DOUBLING OF A DIODE-PUMPED CR-LISAF LASER
    FALCOZ, F
    BALEMBOIS, F
    GEORGES, P
    BRUN, A
    RYTZ, D
    OPTICS LETTERS, 1995, 20 (11) : 1274 - 1276
123