Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

被引：11
|
作者：
McConnell, G
Smith, GL
Girkin, JM
Gurney, AM
Ferguson, AI
机构：
[1] Univ Strathclyde, Ctr Biophoton, Glasgow G4 0NR, Lanark, Scotland
[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
[3] Univ Strathclyde, Inst Photon, Glasgow G4 0NW, Lanark, Scotland
[4] Univ Strathclyde, Dept Physiol & Pharmacol, Glasgow G4 0NR, Lanark, Scotland
[5] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland
来源：
OPTICS LETTERS | 2003年 / 28卷 / 19期
关键词：
D O I：
10.1364/OL.28.001742
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2. (C) 2003 Optical Society of America.
引用
收藏
页码：1742 / 1744
页数：3
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