Twelve cultivars of barley (Hordeum vulgare L.) from the collection of the Vavilov Institute of Plant Industry, Russian Academy of Agricultural Sciences, were screened by assessing the length of 6-day-old seedlings grown in water culture at 70, 120, and 170 mM NaCl. As a result, two salt-susceptible cultivars, Belogorskii and QB 60.1, and three salt-tolerant cultivars, Elo, Odesskii 115, and Local from Ecuador, were selected, and these cultivars were used in the greenhouse soil-culture experiments. The grain yield of salt-tolerant cultivars was affected by NaCl to a lesser degree than that of the salt-susceptible cultivars. In both cases, soil salinization increased the sodium content in the seedlings as compared to the control plants. Characteristically, salt-susceptible cultivars accumulated more Na+ in their shoots than salt-tolerant cultivars; the reciprocal pattern was found in the roots. Soil salinization decreased K+ content in the shoots of the salt-susceptible cv. Belogorskii as compared to the control, whereas in the most tolerant cv. Local from Ecuador, the potassium content increased.