An efficient template-independent polymerase chain displacement reaction for the detection of Salmonella typhimurium

被引:5
|
作者
Wang, Jing [1 ]
Li, Haigang [1 ]
Li, Tingting [1 ]
Zhang, Ji [2 ]
Ling, Liansheng [1 ]
机构
[1] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, Dept Neurosurg,State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
ROLLING CIRCLE AMPLIFICATION; REACTION RT-PCR; REAL-TIME PCR; NUCLEIC-ACIDS; ISOTHERMAL AMPLIFICATION; SENSITIVE DETECTION; GOLD NANOPARTICLES; FLUOROGENIC PROBES; MICRORNA DETECTION; COMPLEX;
D O I
10.1039/c8ay01625a
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here, we incorporated the tailed tandem repeat gene-specific primertechnique into PCDR (TTR-PCDR), developing a template-independent PCDR which allows a rapid, one-tube reaction. Moreover, this TTR-PCDR was applied to the efficient detection of Salmonella typhimurium.
引用
收藏
页码:4229 / 4232
页数:4
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