An efficient template-independent polymerase chain displacement reaction for the detection of Salmonella typhimurium

被引:5
|
作者
Wang, Jing [1 ]
Li, Haigang [1 ]
Li, Tingting [1 ]
Zhang, Ji [2 ]
Ling, Liansheng [1 ]
机构
[1] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, Dept Neurosurg,State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
ROLLING CIRCLE AMPLIFICATION; REACTION RT-PCR; REAL-TIME PCR; NUCLEIC-ACIDS; ISOTHERMAL AMPLIFICATION; SENSITIVE DETECTION; GOLD NANOPARTICLES; FLUOROGENIC PROBES; MICRORNA DETECTION; COMPLEX;
D O I
10.1039/c8ay01625a
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here, we incorporated the tailed tandem repeat gene-specific primertechnique into PCDR (TTR-PCDR), developing a template-independent PCDR which allows a rapid, one-tube reaction. Moreover, this TTR-PCDR was applied to the efficient detection of Salmonella typhimurium.
引用
收藏
页码:4229 / 4232
页数:4
相关论文
共 50 条
  • [1] Fluorescent xDNA nucleotides as efficient substrates for a template-independent polymerase
    Jarchow-Choy, Sarah K.
    Krueger, Andrew T.
    Liu, Haibo
    Gao, Jianmin
    Kool, Eric T.
    NUCLEIC ACIDS RESEARCH, 2011, 39 (04) : 1586 - 1594
  • [2] Fluorescent xDNA nucleotides as efficient substrates for a template-independent polymerase
    Jarchow-Choy, Sarah K.
    Cho, Younjin
    Krueger, Andrew T.
    Kool, Eric T.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2010, 239
  • [3] Engineering the Activity of a Template-Independent DNA Polymerase
    Milisavljevic, Marija
    Rodriguez, Teresa Rojas
    Carlson, Courtney K.
    Liu, Chang C.
    Tyo, Keith E. J.
    ACS SYNTHETIC BIOLOGY, 2024, 13 (08): : 2492 - 2504
  • [4] Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar Typhimurium
    Lim, YH
    Hirose, K
    Izumiya, H
    Arakawa, E
    Takahashi, H
    Terajima, J
    Itoh, K
    Tamura, K
    Kim, S
    Watanabe, H
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2003, 56 (04) : 151 - 155
  • [5] Detection of Salmonella spp., Salmonella Enteritidis, Salmonella Typhi and Salmonella Typhimurium in cream cakes by polymerase chain reaction (PCR)
    Can, Hayriye Yesim
    Elmali, Mehmet
    Karagoz, Alper
    Oner, Suleyman
    MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, 2014, 70 (11): : 689 - 692
  • [6] Development and use of polymerase chain reaction for the specific detection of Salmonella typhimurium in stool and food samples
    Lin, JS
    Tsen, HY
    JOURNAL OF FOOD PROTECTION, 1999, 62 (10) : 1103 - 1110
  • [7] Use of the polymerase chain reaction for Salmonella detection
    Kwang, J
    Littledike, ET
    Keen, JE
    LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (01) : 46 - 51
  • [8] Detection of Salmonella typhi by polymerase chain reaction
    Zhu, Q.
    Lim, C. K.
    Chan, Y. N.
    Journal of Applied Bacteriology, 80 (03):
  • [9] Use of the polymerase chain reaction for Salmonella detection
    U. States Department of Agriculture, Agricultural Research Service, Roman L. Hruska US Meat Anim. R., Clay Center, NE, United States
    不详
    LETT. APPL. MICROBIOL., 1 (46-51):
  • [10] Rapid detection of Salmonella by polymerase chain reaction
    Riyaz-Ul-Hassan, S
    Verma, V
    Qazi, GN
    MOLECULAR AND CELLULAR PROBES, 2004, 18 (05) : 333 - 339