Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry

被引：3

作者：

Ahn, Jang-Hyuk ^{[2
]}

Jeong, Young-Su ^{[3
]}

Lee, Tae Geol ^{[4
]}

Kim, Young-Pil ^{[1
]}

Kim, Hak-Sung ^{[3
]}

机构：

[1] Hanyang Univ, Dept Life Sci, Seoul 133791, South Korea

[2] Namyang Dairy Co Ltd, R&D Inst, Food Safety Ctr, Gongju 314914, South Korea

[3] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[4] KRISS, Ctr Nanobio Convergence, Taejon 305600, South Korea

来源：

BIOCHIP JOURNAL | 2012年 / 6卷 / 01期

关键词：

Aflatoxin; TOF-SIMS; Quantification; Multiplexed analysis; SELF-ASSEMBLED MONOLAYERS; TOF-SIMS; QUANTITATIVE-ANALYSIS; SURFACE-ANALYSIS; B-1; PROTEIN; COLUMN; ELISA; HPLC; NANOPARTICLES;

D O I：

10.1007/s13206-012-6105-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions ([M+H](+)) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL(-1) This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.

引用

页码：34 / 40

页数：7

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