In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy

被引：0

作者：

Koga, Shigehiro ^{[1
]}

Oshima, Yusuke ^{[2
]}

Hikita, Atsuhiko ^{[2
]}

Sato, Koichi ^{[1
]}

Yoshida, Motohira ^{[1
]}

Yamamoto, Yuji ^{[1
]}

Iimura, Tadahiro ^{[3
]}

Watanabe, Yuji ^{[1
]}

Imamura, Takeshi ^{[2
]}

机构：

[1] Ehime Univ, Sch Med, Gastrointestinal Surg & Surg Oncol, Matsuyama, Ehime 790, Japan

[2] Ehime Univ, Sch Med, Dept Mol Med Pathogenesis, Matsuyama, Ehime 790, Japan

[3] Ehime Univ, Proteo Sci Ctr, Div Bioimaging, Toon, Ehime 7910295, Japan

来源：

REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS VII | 2015年 / 9339卷

关键词：

CEA; cancer imaging; multiphoton microscopy; fluorescent probe;

D O I：

10.1117/12.2079092

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We developed a near infrared fluorophore-conjugated anti-Carcinoembryonic antigen (CEA) antibody for mice bearing tumor of CEA expressing cells, and demonstrated in vivo optical imaging by macro zoom microscopy. In the result, tumors of CEA-expressing cancer cells were specifically detected in vivo. Furthermore, cancer-specific fluorescence images were acquired at subcellular level in vivo by two-photon microscopy. In preclinical applications, the lymph node micrometastasis was also successfully visualized by two-photon microscopy. These results suggest that two-photon excitation microscopy in combination with an immunoconjugated probe could be widely adapted to cancer detection in clinical settings.

引用

页数：4

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