Ectopic expression of a vesicle trafficking gene, OsRab7, from Oryza sativa, confers tolerance to several abiotic stresses in Escherichia coli

被引：0
|
作者：
Peng, Xiaojue [1 ]
Zeng, Xin [1 ]
Ding, Xia [1 ]
Li, Shaobo [1 ]
Yu, Chao [1 ]
Zhu, Youlin [1 ]
机构：
[1] Nanchang Univ, Coll Life Sci, Key Lab Mol Biol & Gene Engn, Nanchang 330047, Peoples R China
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 36期
关键词：
Vesicle trafficking; rice; OsRab7; Escherichia coli; abiotic stresses; LATE ENDOSOME; PROTEIN; RAB7; YEAST; OVEREXPRESSION; APOPTOSIS; INDUCTION; GTPASE; SALT;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Rab7 is a small GTP-binding protein involved in intracellular vesicle trafficking from late endosome to the vacuole. In this study, the gene OsRab7 was isolated from Oryza sativa. Over-expression of OsRab7 gene in Escherichia coli increased the resistance to heat, cold and salt stress. In addition, subcellular localization of OsRab7 protein in E. coli and onion cells all revealed that OsRab7 protein is specifically present in the cytoplasm. These results showed that OsRab7 plays an important role in stress tolerance and probably develop abiotic stress tolerance in E. coli and planta with similar mechanism.
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页码：6941 / 6946
页数：6
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