Quantitative phase-shifting DIC using programmable spatial light modulators

被引:0
|
作者
Nguyen, Tan H. [1 ]
Edwards, Chris [1 ,2 ]
Goddard, Lynford [2 ]
Popescu, Gabriel [1 ]
机构
[1] Univ Illinois, Quantitat Light Imaging Lab, Beckman Inst Adv Sci & Technol, Dept Elect & Comp Engn, Urbana, IL 61801 USA
[2] Univ Illinois, Photon Syst Lab, Micro & Nanotechnol Lab, Dept Elect & Comp Engn, Urbana, IL 61801 USA
来源
QUANTITATIVE PHASE IMAGING | 2015年 / 9336卷
关键词
Quantitative DIC; halo-free; microscopy; programmable spatial light modulator; MICROSCOPY; CONTRAST;
D O I
10.1117/12.2080370
中图分类号
TH742 [显微镜];
学科分类号
摘要
A quantitative phase-shifting Differential Interference Contrast (DIC) system is built using a programmable spatial light modulator (SLM). Our system offers halo-free phase gradient images with low illumination coherence and very good axial sectioning. Results are presented for standard polystyrene micro-beads and live cells.
引用
收藏
页数:5
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