Is the secondary putative RNA-RNA interaction site relevant to GcvB mediated regulation of oppA mRNA in Escherichia coli?

被引：6
|
作者：
Busi, Florent [1 ]
Le Derout, Jacques [1 ]
Cerciat, Marie [1 ]
Regnier, Philippe [1 ]
Hajnsdorf, Eliane [1 ]
机构：
[1] Univ Paris 07, Inst Biol Physicochim, UPR CNRS 9073, F-75005 Paris, France
来源：
BIOCHIMIE | 2010年 / 92卷 / 10期
关键词：
GcvB RNA; hfq; OppA; DppA; E; coli; HFQ; BINDING; DEGRADATION; EXPRESSION; GENES; DPPA;
D O I：
10.1016/j.biochi.2010.06.020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. In this work, we have studied whether the formation of a second interaction site could help stabilize the previously reported GcvB/oppA complex. Several mutations and the full deletion of PS1 were engineered. None of these modifications affected the ability of GcvB to control OppA expression. Therefore the second, putative, interaction site appears to be unnecessary for the regulatory function of GcvB with regard to its oppA target mRNA. (C) 2010 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：1458 / 1461
页数：4
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