Immunogenicity of plasmids encoding T and B cell epitopes of foot-and-mouth disease virus (FMDV) in swine

被引:23
|
作者
Cedillo-Barron, L
Foster-Cuevas, M
Cook, A
Gutiérrez-Castañeda, B
Kollnberger, S
Lèfevre, F
Parkhouse, RME
机构
[1] Inst Anim Hlth, Pirbright Lab, Pirbright GU24 0NF, Surrey, England
[2] INRA, Virol & Immunol Mol, F-78350 Jouy En Josas, France
[3] Gulbenkian Inst Sci, P-2780156 Oeiras, Portugal
[4] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
[5] IPN, ESM, Secc Grad & Invest, Mexico City 11360, DF, Mexico
[6] Inst Mol Med, Dept Human Immunol, Oxford OX3 9DS, England
关键词
DNA immunization; foot-and-mouth disease; vaccines;
D O I
10.1016/S0264-410X(03)00453-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In this work, we have investigated the immune response in pigs to two recombinant plasmids containing immunodominant neutralizing antibody epitopes of foot-and-mouth disease virus structural protein (VP1) coexpressed with viral non-structural proteins as a source of T cell epitopes. The plasmid pcDNA3.1/3D 15 contained a sequence coding for the 3D polymerase upstream of a sequence coding for peptide FMDV15, a peptide derived from VP1, previously shown to stimulate protective immunity to foot-and-mouth disease virus (FMDV), that consisted of the carboxy terminal peptide [VP1(200-213)] linked by ProProSer to the "loop" peptide [VP1(143-160)] and terminating in CysGly. The plasmid, pcDNA3.1/2B15 contained a sequence coding for the non-structural protein 2B, and the same FMDV15 peptide sequence. Pigs injected with both constructs showed antibody and T cell responses to 3D and 213, but not to the FMDV15 peptide. Additionally, delayed type hypersensitivity responses were observed in some cases to both 3D or 2D and to FMDV virus. Finally, no protection was seen against FMDV infection in animals immunized with either of the two FMDV DNA constructs. The additional co-immunization of plasmids encoding for GMCSF did not result in any significant change in the immune responses to the plasmids encoding for FMDV. This work gives some optimism for the construction of a DNA vaccine for FMDV in the future. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4261 / 4269
页数:9
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