Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

被引：10
|
作者：
Patzak, J. [1 ]
Matousek, J. [2 ]
机构：
[1] Hop Res Inst Co Ltd, CZ-43846 Zatec, Czech Republic
[2] Acad Sci Czech Republic, Ctr Biol, Intitute Plant Mol Biol, CZ-37005 Ceske Budejovice, Czech Republic
来源：
BIOLOGIA PLANTARUM | 2011年 / 55卷 / 04期
关键词：
cluster analysis; dendrogram; Humulus lupulus; Humulus japonicus; simple sequence repeat; HUMULUS-LUPULUS L; GENETIC DIVERSITY; MOLECULAR METHODS; NORTH-AMERICAN; WILD HOPS; DIFFERENTIATION; VARIABILITY; STS;
D O I：
10.1007/s10535-011-0183-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.
引用
收藏
页码：761 / 765
页数：5
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