Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode

被引：26

作者：

Yin, Bing ^{[1
]}

Yuan, Ruo ^{[1
]}

Chai, Yaqing ^{[1
]}

Chen, Shihong ^{[1
]}

Cao, Shurui ^{[1
]}

Xu, Yang ^{[1
]}

Fu, Ping ^{[1
]}

机构：

[1] SW Univ, Coll Chem & Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chitosan; glucose biosensor; glucose oxidase; layer-by-layer assembly; Prussian blue;

D O I：

10.1007/s10529-007-9534-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 mu M to 1.6 mM with a detection limit of 3.1 mu M glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.

引用

页码：317 / 322

页数：6

共 50 条

[31] Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode
HaiLing Chen
Li Zhao
Xi Chen
ZhiXia Zhuang
XiaoRu Wang
Science in China Series B: Chemistry, 2009, 52 : 1128 - 1135
[32] Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode
CHEN HaiLing1
2 State Key Laboratory of Marine Environmental Science
Science in China(Series B:Chemistry), 2009, (08) : 1128 - 1135
[33] Reagent-less amperometric glucose biosensor based on a graphite rod electrode layer-by-layer modified with 1,10-phenanthroline-5,6-dione and glucose oxidase
Kausaite-Minkstimiene, Asta
Simanaityte, Ruta
Ramanaviciene, Almira
Glumbokaite, Laura
Ramanavicius, Arunas
TALANTA, 2017, 171 : 204 - 212
[34] Amperometric glucose biosensor based on layer-by-layer films of microperoxidase-11 and liposome-encapsulated glucose oxidase
Graca, J. S.
de Oliveira, R. F.
de Moraes, M. L.
Ferreira, M.
BIOELECTROCHEMISTRY, 2014, 96 : 37 - 42
[35] AN AMPEROMETRIC GLUCOSE ELECTRODE BASED ON ADSORBED GLUCOSE-OXIDASE ON PALLADIUM GOLD MODIFIED GRAPHITE
JONSSON, G
GORTON, L
ANALYTICAL LETTERS, 1987, 20 (06) : 839 - 855
[36] An Amperometric Immunosensor Based on a Polyelectrolyte/Nano Gold particle Layer-by-layer Assembly Modified Electrode for the Determination of clenbuterol in meat
Gao, Lijuan
Gan, Ning
Hu, Futao
Cao, Yuting
Zheng, Lei
HIGH PERFORMANCE STRUCTURES AND MATERIALS ENGINEERING, PTS 1 AND 2, 2011, 217-218 : 1793 - +
[37] Functional Layer-By-Layer Design of Xerogel-Based First-Generation Amperometric Glucose Biosensors
Poulos, Nicholas G.
Hall, Jackson R.
Leopold, Michael C.
LANGMUIR, 2015, 31 (04) : 1547 - 1555
[38] Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin
Chai, Rong
Yuan, Ruo
Chai, Yaqin
Ou, Chaofeng
Cao, Shurui
Li, Xuelian
TALANTA, 2008, 74 (05) : 1330 - 1336
[39] Layer-by-layer assembly of 1,4-diaminoanthraquinone and glucose oxidase
Berchmans, S
Sathyajith, R
Yegnaraman, V
MATERIALS CHEMISTRY AND PHYSICS, 2003, 77 (02) : 390 - 396
[40] Multilayered construction of glucose oxidase on gold electrodes based on layer-by-layer covalent attachment
Zhang, SX
Yang, WW
Niu, YM
Sun, CQ
ANALYTICA CHIMICA ACTA, 2004, 523 (02) : 209 - 217

← 1 2 3 4 5 →