Formulation, biopharmaceutical evaluation and in-vitro screening of polyherbal phytosomes for breast cancer therapy

被引:6
|
作者
Govindaram, Lalitha K. [1 ]
Al Bratty, Mohammed [1 ]
Alhazmi, Hassan A. [1 ]
Kandasamy, Ruckmani [2 ]
Thangavel, Neelaveni [1 ]
Ibrahim, Angum M. [1 ]
Mariya, Gover Antoniraj [2 ]
Kumar, Ponnuchamy [3 ]
机构
[1] Jazan Univ, Coll Pharm, Dept Pharmaceut Chem, Jazan, Saudi Arabia
[2] Anna Univ, Univ Coll Engn, Deaprtment Pharmaceut Technol, Tiruchirapalli, India
[3] Alagappa Univ, Dept Anim Hlth & Management, Food Chem & Mol Canc Biol Lab, Karaikkudi, Tamil Nadu, India
关键词
Phytosomes; Curcuma longa; Matricaria chamomilla; Withania somnifera; breast cancer; MCF-7 cell lines; MDA MB 231 cell lines; CURCUMIN; COMBINATION; DOXORUBICIN; SUPPRESSES; ABSORPTION; APIGENIN; EXTRACT;
D O I
10.1080/03639045.2022.2138911
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Saudi Arabia has a rich culture of folk medicines and three such common herbs used by Saudi people for therapy of breast cancer are Turmeric (Kurkum) Curcuma longa, Chamomile (Babunaj) Matricaria chamomilla, and Aswaghantha (Aswaghadh) Withania somnifera. Hence, the present study aims to develop a polyherbal phytosome formulation by thin film hydration technique with a synergistic anti-cancer effect for the treatment of breast cancer. The phytosomes were standardized for their phytoconstituents by HPTLC and showed the best optimal properties with a mean vesicle diameter of less than 200 nm, zeta potential in the range of -24.43 to -35.70 mV, and relatively integrated structure with fairly uniform size on TEM. The in vitro MTT assay on MCF-7 breast cancer cell lines and MDA MB 231 breast adenocarcinoma cell lines was carried out. MTT assay on MCF-7 breast cancer cell lines indicated that plant extract-loaded phytosomes exhibited enhanced cytotoxic effects at IC50 values. of 55, 50, 45, 52, 42, 44, and 20 mu g/mL compared to the extracts of C. longa, M. chamomilla, W. somnifera, and their combined extracts (80, 82, 74, 60, 70, 60, and 35 mu g/mL respectively). Moreover, intracellular reactive oxygen species production was found to be higher for phytosomes treated cells at respective IC50 concentrations when compared to extracts. Overall, the developed polyherbal phytosomes were found to be effective and afford synergistic effects for breast cancer therapy.
引用
收藏
页码:552 / 565
页数:14
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