Long-term in vitro cultivation of Borrelia miyamotoi

被引：29

作者：

Margos, Gabriele ^{[1
,2
]}

Stockmeier, Sylvia ^{[1
,2
]}

Hizo-Teufel, Cecilia ^{[1
,2
]}

Hepner, Sabrina ^{[2
]}

Fish, Durland ^{[3
]}

Dautel, Hans ^{[4
]}

Sing, Andreas ^{[1
]}

Dzaferovic, Eldina ^{[2
]}

Rieger, Melissa ^{[2
,5
]}

Jungnick, Sabrina ^{[2
,5
]}

Binder, Katrin ^{[2
]}

Straubinger, Reinhard K. ^{[5
]}

Fingerle, Volker ^{[1
,2
]}

机构：

[1] Bavarian Hlth & Food Safety Author, D-85764 Oberschleissheim, Germany

[2] German Natl Reference Ctr Borrelia, Oberschleissheim, Germany

[3] Yale Univ, Sch Publ Hlth, New Haven, CT USA

[4] IS Insect Serv GmbH, Berlin, Germany

[5] Univ Munich, Munich, Germany

来源：

TICKS AND TICK-BORNE DISEASES | 2015年 / 6卷 / 02期

关键词：

Borrelia miyamotoi; Relapsing fever spirochetes; In vitro cultivation; LYME-DISEASE; MENINGOENCEPHALITIS; HUMANS; VECTOR; TICK;

D O I：

10.1016/j.ttbdis.2014.12.001

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2. (C) 2014 Elsevier GmbH. All rights reserved.

引用

页码：181 / 184

页数：4

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