A Novel Fluoroimmunoassay for Detecting Ruscogenin with Monoclonal Antibodies Conjugated with CdSe/ZnS Quantum Dots

被引:11
|
作者
Zhang, Hongwei [1 ]
Xu, Tao [1 ]
Gao, Lan [1 ]
Liu, Xiufeng [1 ]
Liu, Jihua [1 ,2 ]
Yu, Boyang [2 ]
机构
[1] China Pharmaceut Univ, Jiangsu Prov Key Lab TCM Evaluat & Translat Dev, Nanjing 211198, Jiangsu, Peoples R China
[2] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 211198, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
fluorescent immunosorbent assay; ruscogenin; monoclonal antibody; quantum dots; tissue distribution; INDUCED PULMONARY-HYPERTENSION; AFLATOXIN B-1; RADIX; SULFAMETHAZINE; INVOLVEMENT; MEDICINES; RESIDUE; INJURY; ELISA; MILK;
D O I
10.3390/molecules22081250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ruscogenin (RUS) is a steroidal sapogenin found in Ruscus aculeatus and Ophiopogon japonicus with several pharmacological activities. In the work reported herein, a novel method termed competitive fluorescence-linked immunosorbent assay (cFLISA) based on monoclonal antibodies (mAbs) coupled with quantum dots (QDs) was developed for the quick and sensitive determination of RUS in biological samples. The mAbs against RUS were conjugated with CdSe/ZnS QDs by the crossing-linking reagents and an indirect cFLISA method was developed. There was a good linear relationship between inhibition efficiency and logarithm concentration of RUS which was varied from 0.1 to 1000 ng/mL. The IC50 and limit of detection (LOD) were 9.59 ng/mL and 0.016 ng/mL respectively, which much lower than the enzyme-linked immunosorbent assay (ELISA) method. The recoveries in plasma and tissues were ranged from 82.3% to 107.0% and the intra-and inter-day precision values were below 15%. The developed cFLISA has been successfully applied to the measurement of the concentrations of RUS in biological samples of rats, and showed great potential for the tissue distribution study of RUS. The cFLISA method may provide a valuable tool for the analysis of small molecules in biological samples and such an approach could be applied to other natural products.
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页数:14
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