Optimization of solid-state medium for the production of inulinase by Aspergillus ficuum JNSP5-06 using response surface methodology

被引:12
|
作者
Chen, Han-Qing [2 ]
Chen, Xiao-Ming [1 ]
Chen, Tian-Xiang [1 ]
Xu, Xue-Ming [1 ]
Jin, Zheng-Yu [1 ]
机构
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Anhui, Peoples R China
关键词
Response surface methodology; Optimization; Aspergillus ficuum JNSP5-06; Inulinase production; Solid-state fermentation; CULTURE-CONDITIONS; JERUSALEM-ARTICHOKE; FERMENTATION; PARAMETERS; DESIGN; ACID;
D O I
10.1016/j.carbpol.2011.04.044
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In the present study, response surface methodology (RSM) was employed to optimize the composition of medium based on Plackett-Burman design and Box-Behnken design for the production of inulinase by Aspergillus ficuum JNSP5-06 using solid-state fermentation (SSF). Inulin, NH4H2PO4 and corn steep liquor were found to have significant effects on inulinase production by the Plackett-Burman design. The concentrations of the three compositions above were further optimized using a Box-Behnken design. The results showed that the final concentration of medium optimized with RSM was 11.47% inulin, 0.76% NH4H2PO4 and 5.71% corn steep liquor by employing wheat bran as the solid substrate. Under optimized medium, the average inulinase activity reached 205.63 U/g initial dry substrate. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:249 / 254
页数:6
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