Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

被引：23
|
作者：
Weber, Michael [1 ]
Mickoleit, Michaela [1 ]
Huisken, Jan [1 ]
机构：
[1] Max Planck Inst Mol Cell Biol & Genet, Huisken Lab, Dresden, Germany
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2014年 / 84期
关键词：
Developmental Biology; Issue; 84; zebrafish; Danio rerio; light sheet microscopy; Selective Plane Illumination Microscopy; sample mounting; time lapse microscopy; long-term imaging;
D O I：
10.3791/51119
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.
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页数：8
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