Mixed Carbonates as Useful Substrates for a Fluorogenic Assay for Lipases and Esterases

被引：15

作者：

Zadlo, Anna ^{[1
]}

Koszelewski, Dominik ^{[1
]}

Borys, Filip ^{[2
]}

Ostaszewski, Ryszard ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Organ Chem, PL-01224 Warsaw, Poland

[2] Warsaw Univ Technol, Fac Chem, Dept Technol & Biotechnol Med, PL-00664 Warsaw, Poland

来源：

CHEMBIOCHEM | 2015年 / 16卷 / 04期

关键词：

carbonates; enzyme catalysis; fluorescent probes; high-throughput screening; hydrolases; ENANTIOSELECTIVE CATALYSTS; KINETIC RESOLUTION; FLUORESCENCE ASSAY; ENZYME ASSAYS; THROUGHPUT; HYDROLYSIS; HYDROLASES; PROBES;

D O I：

10.1002/cbic.201402528

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mixed carbonates of 7-hydroxy-4-methylcoumarin were shown to be a new class of probe for fluorogenic assays. They are promising substrates for fingerprinting enzyme hydrolytic activity, and proved particularly useful because of the low level of nonspecific degradation and ease of synthesis. They are highly relevant for screening lipase and esterase libraries. These advantages make umbelliferyl carbonates highly suitable substrates for high-throughput screening. Moreover, we report the use of chiral fluorogenic carbonates as enantiopreference probes.

引用

页码：677 / 682

页数：6

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