Development of microsatellite markers for muskellunge (Esox masquinongy) and cross-species amplification in two other esocids

被引:6
|
作者
Reading, BJ [1 ]
Wills, PS
Heidinger, RC
Heist, EJ
机构
[1] So Illinois Univ, Fisheries & Illinois Aquaculture Ctr, Carbondale, IL 62901 USA
[2] So Illinois Univ, Dept Zool, Carbondale, IL 62901 USA
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 03期
关键词
Esox; grass pickerel; microsatellite; muskellunge; northern pike;
D O I
10.1046/j.1471-8286.2003.00479.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the development of seven polymorphic microsatellite loci in muskellunge (Esox masquinongy) using an unenriched subgenomic library. Polymorphic loci exhibited 2-11 alleles with observed heterozygosities ranging from 0.190 to 0.917 (n = 24). All seven loci amplified by their respective primer pairs resulted in monomorphic products in northern pike (E. lucius) whereas three loci amplified monomorphic products in grass pickerel (E. americanus americanus); these results imply conservation of flanking sequence but loss of polymorphism between these closely related species. Only one of six microsatellite primers developed in a previous study in northern pike amplified polymorphic products in muskellunge.
引用
收藏
页码:447 / 449
页数:3
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