Two-photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications

被引：104
|
作者：
Clapp, Aaron R.
Pons, Thomas
Medintz, Igor L.
Delehanty, James B.
Melinger, Joseph S.
Tiefenbrunn, Theresa
Dawson, Philip E.
Fisher, Brent R.
O'Rourke, Brian
Mattoussi, Hedi
机构：
[1] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA
[2] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA
[3] USN, Res Lab, Div Elect Sci & Technol, Washington, DC 20375 USA
[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[5] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[6] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[7] MIT, Dept Chem, Cambridge, MA 02139 USA
[8] Johns Hopkins Univ, Sch Med, Inst Mol Cardiobiol, Baltimore, MD 21205 USA
来源：
ADVANCED MATERIALS | 2007年 / 19卷 / 15期
关键词：
D O I：
10.1002/adma.200602036
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Nonradiative fluorescence resonance energy transfer (FRET) between a luminescent quantum dot (QD) donor and a proximal dye brought in close proximity of the QD surface via conjugation with a dye-labeled peptide (or a protein) is shown (see figure). The system is excited with near IR irradiation (well below the absorption band of the QD), via a fast two-photon process, which produces a FRET signal with very low background contribution due to a substantially reduced nonlinear direct excitation of the dye.
引用
收藏
页码：1921 / +
页数：7
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