Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction

被引：22

作者：

Goldenberg, Simon D. ^{[1
,2
]}

Gumban, Maria ^{[3
]}

Hall, Anthony ^{[3
]}

Patel, Amita ^{[1
]}

French, Gary L. ^{[1
,2
]}

机构：

[1] Guys & St Thomas NHS Fdn Trust, Directorate Infect, London SE1 7EH, England

[2] Kings Coll London, Dept Infect Dis, London SE1 9RT, England

[3] GSTS Pathol, London SE1 7EH, England

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 70卷 / 03期

关键词：

Clostridium difficile; Glutamate dehydrogenase; Polymerase chain reaction; Laboratory techniques and procedures; REAL-TIME PCR; DIAGNOSTIC ALGORITHM; ENZYME-IMMUNOASSAY; 2-STEP ALGORITHM; COMMON ANTIGEN; TOXIN; TESTS; ASSAY; CULTURE;

D O I：

10.1016/j.diagmicrobio.2011.03.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. Crown Copyright (c) 2011 Published by Elsevier Inc. All rights reserved.

引用

页码：417 / 419

页数：3

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