Determination of caffeine content in coffee using Fourier transform infra-red spectroscopy in combination with attenuated total reflectance technique: a bioanalytical chemistry experiment for biochemists

被引：33
|
作者：
Singh, BR [1 ]
Wechter, MA [1 ]
Hu, YH [1 ]
Lafontaine, C [1 ]
机构：
[1] Univ Massachusetts, Dept Biochem & Chem, N Dartmouth, MA 02747 USA
来源：
BIOCHEMICAL EDUCATION | 1998年 / 26卷 / 03期
关键词：
D O I：
10.1016/S0307-4412(98)00078-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this report we describe an experiment to estimate the amount of caffeine present in common beverages with the use of Fourier transform infra-red spectroscopy (FTIR), using an attenuated total reflectance accessory as a sampling system. The absorbance band at 1655 cm(-1) was used to estimate caffeine in standards and in the samples. The sensitivity of the technique was 5 ppm. The experiment provides a good demonstration of a bio-analytical technique that can be adopted either for a biochemistry or an analytical chemistry course. (C) 1998 IUBMB. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：243 / 247
页数：5
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