Reference gene selection for quantitative real-time polymerase chain reaction in Populus

被引：123
|
作者：
Xu, Meng [1 ,2 ]
Zhang, Bo [1 ,2 ]
Su, Xiaohua [3 ]
Zhang, Shougong [3 ]
Huang, Minren [1 ,2 ]
机构：
[1] Nanjing Forestry Univ, Jiangsu Key Lab Poplar Germplasm Enhancement & Va, Nanjing 210037, Peoples R China
[2] Nanjing Forestry Univ, Key Lab Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China
[3] Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2011年 / 408卷 / 02期
关键词：
RT-PCR; EXPRESSION; GENOME; RNA;
D O I：
10.1016/j.ab.2010.08.044
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：337 / 339
页数：3
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