Biochemical characterization of bovine αs1-casein F and genotyping with sequence-specific primers

被引：5
|
作者：
Prinzenberg, EM
Anglade, P
Ribadeau-Dumas, B
Erhardt, G
机构：
[1] Univ Giessen, Inst Tierzucht & Haustiergenet, D-35390 Giessen, Germany
[2] INRA, Unite Rech Biochim & Siructure Prot, F-78352 Jouy En Josas, France
来源：
JOURNAL OF DAIRY RESEARCH | 1998年 / 65卷 / 02期
关键词：
D O I：
10.1017/S0022029997002690
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Bovine alpha(s1)-casein F (alpha(s1)-CN F) was found in a genetic resource of Deutsches Schwarzbuntes Niederungsrind cows at a frequency of 0.009. Biochemical characterization of this new variant was obtained by automated sequencing of reversed-phase HPLC-separated tryptic peptides of alpha(s1)-CN F and alpha(s2)-CN B. alpha(s1)-CN F was found to be a subtype of alpha(s1)-CN B with a single aminoacid substitution (SerP/Leu) in position 66. DNA sequencing revealed a C/T transition in position 8418 of the gene. Sequence-specific primers were designed to perform an allele-specific polymerase chain reaction for detection of alpha(s1) Cn(F). Typing of artificial insemination sperm samples included in the genetic resource sperm pool identified one sire heterozygous for alpha(s1) Cn(F).
引用
收藏
页码：223 / 231
页数：9
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