A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus

被引:17
|
作者
Xu, LiJing [1 ,2 ]
Wang, HeXiang [1 ,2 ]
Ng, TziBun [3 ]
机构
[1] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
[2] China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China
[3] Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
来源
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年
关键词
FUNGUS PLEUROTUS-OSTREATUS; RIBOSOME-INACTIVATING PROTEIN; HUMAN-IMMUNODEFICIENCY-VIRUS; FRUITING BODIES; EDIBLE MUSHROOM; PANUS-TIGRINUS; PURIFICATION; RIBONUCLEASE; EDODES; PANUS-TIGRINUS-8/18;
D O I
10.1155/2012/536725
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC50 = 2.4 mu M) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion exchangers. About 95-fold purification was achieved with a 25.9% yield of the enzyme. The procedure resulted in a specific enzyme activity of 76.6 U/mg. Its N-terminal amino acid sequence was GIPDLHDLTV, which showed little similarity to other mushroom laccase and other Lentinus tigrinus strain laccase. Its characteristics were different from previously reported laccase of other Lentinus tigrinus strain. Maximal laccase activity was observed at a pH of 4 and at a temperature of 60 degrees C, respectively. This study yielded the information about the potentially exploitable activities of Lentinus tigrinus laccase.
引用
收藏
页数:7
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